Glycerol dehydrogenase from Cellulomonas sp. NT3060: Purification and characterization.
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چکیده
منابع مشابه
Purification and Characterization of Cellulose Degrading Enzyme from Newly Isolated Cellulomonas Sp
National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, College of Land and Environment, Shenyang Agricultural University, Shenyang, Liaoning 110866, China * Department of Biotechnology, University of Sargodha, Sargodha 40100, Pakistan ** English Teaching Department, Shenyang Agricultural University, Shenyang 110866 Liaoning, China *** Bioscience and Biotechn...
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Cellulase production by a Cellulomonas sp., isolated in 1985 from forest humus soil along the border of the Caspian Sea in Iran, was investigated. This strain secreted endo-and exo-cellulases in the culture medium, but some of the enzymes produced remained cell membrane bound. Cell bound enzymes were released by various treatments. The highest amount of endo-glucanase (up to 95%) and exo-gl...
متن کاملPurification and Characterization of Glycerol-3-phosphate Dehydrogenase (Flavin-linked) from Rat Liver
We have purified the membrane-intrinsic glycerol3-phosphate dehydrogenase from both normal and hyperthyroid rat liver mitochondria by extraction with Triton X-100, hydrophobic affinity chromatography, ion exchange chromatography, gel filtration, and FAD-linked Sepharose 4B affinity chromatography. The yields in both cases were over 20‘70, and purification ranged from 800to 650-fold in mitocho...
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Glycerol dehydrogenase from Aerobacfer aerogenes has been partially purified. Kinetic constants for the substrates are reported and an Ordered Bi Bi mechanism based on product inhibition studies is presented. An investigation of the kinetics of monovalent cation activation shows that substrate affinities, particularly those for glycerol and dihydroxyacetone, are affected by monovalent cations, ...
متن کاملPartial Purification and Characterization of the Recombinant Benzaldehyde Dehydrogenase from Rhodococcus ruber UKMP-5M
Background: Benzaldehyde dehydrogenase (BZDH) is encoded by the xylC that catalyzes the conversion of benzaldehyde into benzoate in many pathways such as toluene degradation. Objectives: In this study, the xylC gene from Rhodococcus ruber UKMP-5M was expressed in Escherichia coli, purified, and characterized.Materials and Methods: The xylC was amplified and cloned in E. coli. The re...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1982
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.46.2333